Measurement of NET formation in vitro and in vivo by flow cytometry

mpo netNETs were quantified by measuring MPO-DNA complex using an ELISA. The results showed that MPO-DNA complex levels were lower with advancing age,Neutrophil extracellular traps (NETs) are extracellular chromatin fibers adorned with antimicrobial proteins, such as myeloperoxidase (MPO), which are extruded from activated neutrophils.